Preparing Agarose for Gel Electrophoresis
10 Steps to Prepare Agarose Gel
This semester, my lab partners and I made many Agarose Gels in the Molecular Biology Lab. I’d like to share the process step-by-step to make it easy for you to make your own Agarose Gel. Of course, if you haven’t done so already. The process wasn’t clear to me at first, but I got the hang of it when I attempted to do it by myself multiple times.
|Gel Electrophoresis Unit|
- Pour 50 mLs of 1x TAE buffer in a graduated cylinder.
- Weigh 0.5 grams of Agarose.
- Place the Agarose in a clean Erlenmeyer Flask.
- Pour the 50 mLs of TAE in the flask.
- Heat the solution in a microwave for 30 seconds. Reheat it for another 15 seconds if the solute (Agarose) didn’t melt.
- Allow the solution to cool for 3-5 minutes.
- Add 3 microliters of GELRED Nucleic Acid Stain (10,000x in DMSO). Don’t forget to add the GelRed if you want to see the fragments using UV light.
- Pour the solution in the Electrophoresis Unit.
- Insert the comb in the tray. The comb will make the wells in the Agarose Gel
- Leave the mixture in the Unit for 20 minutes to solidify.